David I. Roper

David Roper has a background in microbial biochemistry and was awarded an MRC Career Development Award fellowship at the University of York prior to taking an academic position at the University of Warwick, where he has now Professor of Biochemistry. The Roper group is focused on the way in which pathogenic bacteria make their cell walls grow, how that is related to the ways in which they divide and reproduce and also the relationship of these processes to the discovery and resistance to antibiotics. The Roper group uses interdisciplinary approaches from basic microbiology to molecular structure determination by X-ray crystallography, relating enzyme and protein mechanism to structure and function at a cellular level. Our research encompasses both fundamental and translation approaches including assay development and drug discovery approaches. In addition, his research group uses a number of approaches to reengineer pathway intermediates as chemical probes, substrates and inhibitors to allows novel insight to the biology underpinning these pathways which may lead to biotechnological exploration and exploitation. This includes the design and synthesis of chemical mimetic small molecule probes for mechanistic and translational studies. 1. **Peptidoglycan Biosynthesis**: The majority of my work is concentrated on understanding the molecular mechanisms that underpin the biosynthesis of peptidoglycan pathogens with particular emphasis on the way in which cell shape is determined and controlled by RodA-PBP and the wider elongasome. This involved coordination of processes from inside to outside the cell membrane** ** 2. **Bacteria Cell division: **Cell division is the largest morphological change that any cell undergoes and has common elements in all forms of life. In bacteria this is controlled by a dynamic complex of proteins, many of which are located at or in the cell membrane making their study and analysis challenging. FtsZ and the proteins which interact are of key interest in that respect. 3. **New antibacterial drug discovery:** Our studies on cell division and their linkage to peptidoglycan synthesis has an intimate connection to the targets of existing and potentially future antimicrobial drugs. We are investigating a number of these including the potential of the lipid II polymerization process as a target for such drugs. 4. **Molecular Mechanisms of Antibiotic resistance.** We are interested in the molecular mechanisms of antibiotic resistance, in relation to the biosynthesis of the cell wall including how Serine Threonine Kinases may modulate cell wall biosynthesis to adapt to antibiotic stress.

Kavaš, Vid, Contreras-Martel, Carlos, Pajk, Stane, Knez, Damijan, Martins, Alexandre, Gould, Thomas A., Roper, David I., Zdovc, Irena, Dessen, Andréa, Hrast Rambaher, Martina and Gobec, Stanislav (2026) <a href="https://wrap.warwick.ac.uk/id/eprint/197299/"><em>Structure–activity relationship and crystallographic study of new monobactams.</em></a> Journal of Medicinal Chemistry, 69 (4). pp. 3887-3901. doi:<a target="_blank" href="https://doi.org/10.1021/acs.jmedchem.5c02427">10.1021/acs.jmedchem.5c02427</a> ISSN 0022-2623. Evans, Adam, Kelly, Bethan, Sridhar, Pooja, Rothnie, Alice J., Pollock, Naomi L., Ireland, Philip M., Roper, David I. and Dafforn, Tim R. (2026) <a href="https://wrap.warwick.ac.uk/id/eprint/195763/"><em>RePol : a high‐throughput screen for optimizing membrane protein so