Daniel Shiwarski

Dr. Shiwarski obtained a combined undergraduate degree from Bucknell University in cell biology and biochemistry, a Ph.D. in Biological Sciences and Neuroscience from Carnegie Mellon University and the Center for Neural Basis of Cognition, and completed a Postdoctoral fellowship in Biomedical Engineering at Carnegie Mellon University in cellular biomechanics and tissue engineering. Dr. Shiwarski has a broad background in cell biology, cancer research, and tissue engineering with specific expertise in G-protein coupled receptor trafficking, quantitative cellular biomechanics, physiology of bimolecular condensates and phase separation, advanced fluorescence imaging, 3D bioprinting, and computational image analysis. Dr. Shiwarski’s doctoral research at Carnegie Mellon University was focused on investigating how opioid receptor membrane trafficking events influence pain inhibition and opioid addiction using advanced live cell fluorescence imaging techniques to track exocytic and endocytic events. During his postdoctoral research fellowship in Dr. Adam Feinberg’s lab Dr. Shiwarski worked on three distinct projects i) development of a fluorescence-based strain biosensor to track real-time microscopic changes in cell and tissue biomechanics, ii) co-development of a novel tissue engineering platform, FRESH, that lead to 3D bioprinting of unmodified collagen into functional components of the human heart and the development of collagen-based tissue microfluidics, and iii) the generation of opensource hardware and software for tissue engineering and cellular biomechanics evaluation. Research Interests: Biomedical engineering Biochemistry and cell biology Medical physiology Materials engineering Medical biotechnology Clinical sciences

Comber, E.M., Roberts, K.G., Joyce, I.M., Palchesko, R.N., Shiwarksi, D.J., Ren, X., Feinberg, A.W., & Cook, K.E. (2026). A Fully Biological Gas-Exchange Membrane toward a Biofabricated, Booster Lung. ACS Biomater Sci Eng , 12(5), 2746-2757.American Chemical Society (ACS). doi: 10.1021/acsbiomaterials.6c00046 . Pereiratavares, A.J., Aranda-Michel, L., Hahn, S., Kaufman, O., Coffin, B.D., Ashraf, S.F., Szafron, J.M., Straub, A.C., & Shiwarski, D.J. (2026). Pressure myography and cardiac flow simulator for mechanical characterization of native and engineered blood vessels. DEVICE , 4(2).Elsevier. doi: 10.1016/j.device.2025.100996 . Hudson, A.R., Shiwarski, D.J., Kramer, A.J., & Feinberg, A.W. (2025). Enhancing Viability in Static and Perfused 3D Tissue Constructs Using Sacrificial Gelatin Mi